JCS -- Lorger and Moelling 119 (16): 3385 Figure IG11

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Fig. 11. AF6i3 C-terminus in Ca²⁺ switch assay. (A) Cell monolayers of the control cells and control or AF6i3-knockdown cells expressing the HA-tagged AF6i3 CT prior to assay (top) and 2 hours after switch to high Ca²⁺ concentration (bottom). Green, anti-AF6 antibody; red, anti-HA antibody. Bar, 20 µm. (B) Cells shown in A 2 hours after switch to high Ca²⁺ concentration, stained with anti-ß-catenin antibody. Bar, 20 µm.