JCS -- Lorger and Moelling 119 (16): 3385 Figure IG5

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Fig. 5. AF6i3 knockdown reduces E-cadherin-dependent adhesion in dissociation assay. (A) Light-microscopy images of cells trypsinised in the presence of 1 mM Ca²⁺ (top) or 1 mM EDTA (bottom) and dissociated by pipetting. Bar, 0.125 mm. (B) Graphic representation of the extent of cell dissociation, represented by index N_TC/N_TE. N_TC and N_TE correspond to the total particle number after treatment of cells in the presence of Ca²⁺ or EDTA, respectively. Values for ±s.d. were derived from three independent experiments.