Fig. 4. Cytoskeletal rearrangements in migrating HEK-293/uPAR-25 cells. (A,B) HEK-293/uPAR-25 cells were seeded onto a glass slide and allowed to migrate towards 1 nM uPA 1-158 or diluents (Control) for 4 hours in a Dunn chamber (Allen et al., 1998). At the end of the incubation, cells were stained with Rhodamine-phalloidin, observed under an inverted fluorescence microscope and analysed as described in Materials and Methods. Original magnification, x400.