Fig. 2. Sedimentation shows reduction of motility. Trypanosomes were incubated in cuvettes and optical density was measured before and after mixing. The recipient cell line 29-13 was used as a negative control and the PFR2 RNAi-mutant snl-2 as positive control. RNAi mutant cell lines were induced for 2 days and grown with (full lines) or without (dotted lines) shaking. Reduction in cell motility was severe in both conditions.