Fig. 3. Antibodies to TN-R FNIII domains 4-A neutralize the inhibitory effect of aggrecan on TN-R-induced morphological changes. Tectal cells were cultivated on immobilized contactin 1 and treated for 6 hours with TN-R (10 µg/ml), aggrecan (20 µg/ml) and Fab fragments of pAb against TN-R FNIII domains 4-A (500 µg/ml) as indicated. For quantitative analysis see Fig. 2. The addition of antibodies against TN-R FNIII domains 4-A has no effect on TN-R-induced microprocess formation and growth cone enlargement, but strongly reduces the blocking effect of aggrecan.