JCS -- Zhang et al. 119 (17): 3491 Figure IG5

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Fig. 5. No effect of PD98059 was seen on pH_i and P-MAPK. (A) Change in pH_i. Eggs were fertilized ( ${blacktriangleup}$ , ${triangleup}$ ) or not ( $bullet$ , ${circ}$ ) in the presence ( ${triangleup}$ , $bullet$ ) or not ( ${blacktriangleup}$ , $bullet$ ) of 50 µM PD98059. PD98059 did not modify the increase in pH_i observed after fertilization, nor did it change the small acidification observed in unfertilized eggs. (B) Changes in the phosphorylation of the ERK-LP detected by western blot using the anti-phosphoMAPK42/44 antibody after fertilization (F) or treatment with 10 mM ammonium (A) or with 10 µM A23187 (I). MAPK was rapidly dephosphorylated after fertilization or activation with the Ca²⁺ ionophore, but no change was observed after treatment with ammonium.