Fig. 3. Histograms showing the time interval between the clathrin and the peaks of GAK and auxilin. (A) Time interval between clathrin and GAK peaks, calculated from the events used in Fig. 2B. (B) Time interval between clathrin and auxilin peaks, calculated from the events used in Fig. 2C. (C) Time interval between clathrin and dynamin1 peaks, calculated from the events used in Fig. 4A. (D) The time interval between clathrin and dynamin2 peaks, calculated from the events in Fig. 4B. In calculating the peak of maximum clathrin fluorescence in the histograms, the fluorescence data were fitted using Microsoft Excel and a moving average of four to reduce noise in the clathrin data.