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Figure 4


Fig. 4. Recruitment of dynamin1 or dynamin2 to CCPs in CV1 cells. Fluorescence intensity of GFP dynamin and mRFP-clathrin observed under TIRF microscopy was analyzed by Metamorph as described in the Materials and Methods. (A) The change in fluorescence intensities of GFP-dynamin1 and mRFP-clathrin as a function of time measured from 25 puncta in 7 cells. (B) The change in fluorescence intensities of GFP-dynamin2 and mRFP-clathrin as a function of time measured from 32 puncta in 5 cells. The zero time point was set to the time at which dynamin reached its maximum.