Fig. 8. Protein lipid overlay obtained using GST-PTEN-like domain of GAK. (A) Binding of GST-PTEN-like domain of GAK to phosolipids was measured using the protein lipid overlay strips. (B) Quantification of the phosolipid overlay. The binding to the phospholipids strip was performed as described in Materials and Methods. The circles along the periphery of the strip indicate the different phospholipids that were tested in the binding assay.