Fig. 5. Contribution of various domains to SR45 mobility and subcellular localization under control conditions, and after treatment with various inhibitors. (A-E) Optical sections from representative FRAP images of protoplasts expressing a deletion mutant. Circles indicate regions that were bleached (A) RS1; (B) RRM; (C) RS2; (D) RS1+RRM; (E) RRM+RS2. Bars, 10 µm. Quantitative FRAP analyses of the SR45-deletion mutants are shown to the right of each set of images. Error bars are ± s.e.m. of at least ten cells. Inset in C shows final recovery levels. Curves in red lines give the theoretical curves a protein of the molecular mass equivalent to the GFP-fusion protein would follow in the absence of any binding (see Materials and Methods). Curves in black give the best-fit curves to the experimental data of an exponential equation determined by the least-square method.