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Figure 2


Fig. 2. Analysis of the region of Pex19p required for re-establishing peroxisome assembly. (A) HA2-Pex19p variants shown in Fig. 1A were expressed in CHO pex19 ZP119 cells: full-length Pex19p (a,b), 1-295 (the same as {Delta}C4) with deletion of four amino acid residues from the C-terminus (c,d), 1-261 ({Delta}C38) (e,f), 1-255 ({Delta}C44) (g,h), and {Delta}256-260 with internal deletion of amino-acid residues 256-260 (i,j). Cells were dual-stained with antisera against PTS1 and Pex14p for verifying peroxisome-restoring activity of Pex19p mutants. (B) HA2-Pex19p deletion mutants in Fig. 1B,C were likewise expressed in ZP119 cells: 12-299 ({Delta}N11) lacking 11 amino-acid residues from the N-terminus (a,b), 24-299 ({Delta}N23) (c,d), {Delta}12-23 (e,f), M12-261 with deletion of 11 and 38 amino acid residues from the N- and C-termini (g,h) and M12-255 (i,j). Cells were stained as in A. Bars, 20 µm.