Fig. 2. Turnover of Tie2 in HUVECs. [³⁵S]methionine and [³⁵S]cysteine labelled HUVECs were washed and incubated at 37°C for the indicated times in either HUVEC medium alone, or containing pre-clustered Ang1 (800 ng/ml), or Ang2 (800 ng/ml). The cells were lysed, Tie2 immunoprecipitated with an anti-Tie2 antibody, and immunoprecipitates resolved by SDS-PAGE. (A) Representative autoradiograph. (B) Semi-logarithmic plot of the relative Tie2 intensities versus time in unstimulated cells (), in cells stimulated with Ang1 () or Ang2 (). Each point on the graph is an average value from three to nine independent experiments.