Fig. 3. Ang1 and Ang2 do not influence Tie2 synthesis. [³⁵S]methionine and [³⁵S]cysteine labelled HUVECs were incubated for various times together with pre-clustered Ang1 (800 ng/ml), or Ang2 (800 ng/ml). Tie2 immunoprecipitates were resolved by SDS-PAGE and the dried gel exposed to a phosphorimager screen. (A) Representative autoradiograph. (B) The intensities of the bands were quantified and plotted against time. Time=12 hours was designated 100%. All other values were expressed as a percentage of the 12-hour time point. Unstimulated cells (), Ang1 (), Ang2 (). Results are mean ± s.e.m. of three independent experiments.