Fig. 4. Blended projection images of NM2A- and NM2B-knockdown cells: effects on cell shape and adhesion. (A) Projection images of NM2A- and NM2B-knockdown and control cells (NM2A-sense treated). The cells have been stained for actin (green), NM2A (for antisense NM2A and sense NM2A) or NM2B (red) and DNA (blue). Sites of interaction between cells are indicated by arrows. (B) A projection image for NM2B-knockdown cells before (0 hours) and after 36 or 72 hours of treatment. The cells have been stained for actin (green), NM2B (red) and DNA (blue). This demonstrates the elongation of the cells following NM2B-knockdown and the change in shape of the lamellipodia. Bar, is 20 µm for all six images.