Fig. 5. NM2B-knockdown affects cell shape and alignment. (A) Low power images show intensity-inverted, fluorescent, low-power images of differentiating myoblast cultures left untreated or treated for 72 hours with sense, scrambled or antisense oligonucleotides for NM2B. These images were taken from less dense regions to show the dramatic increase in cell length that results from NM2B treatment, compared with controls. The cells were co-stained for NM2B and actin filaments. Images were taken using the same settings, and contrast adjusted equally for all the images. (B) Intensity-inverted, fluorescent, high-power images of differentiating myoblast cultures stained for actin after 36 hours of treatment with sense or antisense oligonucleotides to compare cell shape and alignment in groups. The arrows show aligned bipolar cells but, in the antisense treatment, elongated connections between cells (arrowheads) are evident.