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Figure 2


Fig. 2. GFPRab11b localization in stably expressing PtK1 cell lines. Confocal images of living PtK1 cells expressing GFPRab11b-GDP (A,D,G) and GFPRab11b-GTP (B,E,H) visualized by GFP fluorescence. Alexa-568-transferrin (red) in control cells (C,F) and in cells stably expressing the GFPRab11b mutants (D,E,G,H). Live cell images were taken approximately 5 minutes (C-E) or 30 minutes (F-H) after transferrin internalization was induced by a shift from 0°C to 37°C. Corresponding supplemental movies are available for control (supplementary material Movie 1a,b), GFPRab11b-GDP (supplementary material Movie 2a,b) and GFPRab11b-GTP (supplementary material Movie 3a,b) cells.