Fig. 5. Cells transiently expressing ^GFPRab11b mutants exhibit abnormal motility and morphology. The cells had been microinjected with ^GFPRab11b constructs approximately 4 hours before the beginning of the time-lapse, and expressing cells are marked with white dots. Frames are 40 minutes apart. (A) control PtK1 island (see also supplementary material Movie 4) and islands in which some of the cells were microinjected with either the ^GFPRab11b-GTP (B, supplementary material Movie 5) or ^GFPRab11b-GDP (C, supplementary material Movie 6) construct. The expressing cells are marked with a white dot. In C, the cells expressing high levels of ^GFPRab11b-GDP are marked with arrows in the first image and white dots thereafter; the unmarked cell expresses very low levels of ^GFPRab11b-GDP. (D-F) Tracks of migration of control cells (D) and cells expressing ^GFPRab11b-GTP (E) or ^GFPRab11b-GDP (F). Tracks were generated from 6 hour, 4-minutes interval time-lapse movies of cellular islands shown in A-C. Start positions of the cells are marked by gray circles.