Fig. 7. Effects of ^GFPRab11b-GDP expression on the cytoskeleton and on adhesion. (A) ^GFPRab11b-GDP-expressing cells identified by GFP fluorescence; actin (B) and microtubules (C) identified by immunofluorescence; image of the nuclei (blue) identified by DAPI is overlaid onto the microtubule image (C). (D-G) Cell-cell adhesions visualized by indirect immunofluorescence against e-cadherin. (D,F) Pseudocolored overlay images of ^GFPRab11b-GDP (green), E-cadherin (red) and nuclei (blue); (E,G) E-cadherin images of the same cells. (H,I) Confocal micrographs of focal adhesions visualized by indirect immunofluorescence against vinculin. (H) Pseudocolored overlay image of ^GFPRab11b-GDP (green) and vinculin (red); (I) vinculin image of the same cells. Scale bar for all panels, 20 µm.