Fig. 7. Overexpression of Klf5 in the adult epidermis. (a) Hyperkeratosis, follicle occlusion and hyperproliferation after induction of KLF5-transgene in the bi-transgenic adult mice. Hyperproliferation of keratinocytes is mainly observed at the edges of epidermal erosions. Bar, 50 µm. (b) Analysis of expression of 6 integrin and CD34 markers in keratinocytes by flow cytometry. Keratinocytes were isolated from normal and bi-transgenic adult female mice in which Klf5 overexpression was initiated at 4-5 weeks of age. Keratinocytes were stained with CD34-FITC and 6 integrin-PE-Cy5 and examined by flow cytometry. The bi-transgenics 1 and 2 were 3 months old and the bi-transgenic 3 female and the normal control were 5 months old at the time of analysis.