Fig. 1. Nvj1p can be divided into four domains based on sequence homology across the Saccharomyces family. (A) Alignment of S. cerevisiae Nvj1p and its orthologs in closely related and divergent Saccharomyces species. Amino acid sequences were obtained from the Saccharomyces Genome Database (www.yeastgenome.org). The S. kluyveri ortholog of Nvj1p was identified through a tblastn search of contig 02.1344 (accession AACE02000001). Sequences were aligned using the ClustalX program (Thompson et al., 1997). Boxes were drawn around regions of sequence similarity in the Saccharomyces Nvj1p family. (B) Graphical view of residues in Nvj1p bearing similarity or identity across the Saccharomyces Nvj1p family. ClustalX conservation scores were calculated for each column of the alignment in 1A, and residues bearing similarity (50-74% homology, open circles) or near identity (75-100%, shaded diamonds) were aligned onto a linear map of Nvj1p. These residues sorted independently into four regions, as indicated. (C) Kyte-Doolittle hydropathy plot of the four conserved regions of Nvj1p. Region I at the N-terminus shows an area of considerable hydrophobicity, while region II coincides with the membrane-spanning domain of Nvj1p.