Fig. 7. Native levels of Nvj1p are mildly deleterious for growth in tryptophan-depleted media. nvj1- and its isogenic parental (NVJ1⁺) tryptophan auxotrophic strain (trp1) were grown in rich media to log-phase and shifted into SC media containing limiting (15 µg/ml) or depleted (5 µg/ml) concentrations of tryptophan. Cell growth was monitored at 25°C using a Bioscreen C Analyzer (see Materials and Methods). Error bars were calculated from two independent trials performed in duplicate.