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Figure 1


Fig. 1. Nedd4 binds to Cx43 in vitro. (A) Schematic presentation of Cx43 and the GST-fusion proteins used. The four Cx43 transmembrane domains (TM) are shown as black boxes. (B) SDS-PAGE and Coomassie staining of proteins obtained in GST pull-down assays from WB-F344 cell lysates. In pull-down experiments using GST-fusion protein Cx43 CTer, the tight-junction-associated proteins zonula occludens-1 (ZO-1), zonula occludens-2 (ZO-2), and the ubiquitin-protein ligase Nedd4 were identified after nanoESI-MS. (C) SDS-PAGE and immunoblot with an anti-Nedd4 antibody after GST pull-downs from WB-F344 cell lysates.