Fig. 2. Suppression of Aurora B kinase activity inhibits cell division. Aurora transgenic lines were induced with tetracycline (tet), harvested at various time points and analysed by flow cytometry to determine DNA content. (A) Histograms 32 hours post induction showing that cells expressing the Aurora B and C mutants accumulate DNA contents 4N. (B) Line graphs quantifying cells with DNA contents >4N over a 40-hour time course. At t=0 tetracycline was added to the Aurora transgenic lines indicated or, alternatively, ZM1 was added to uninduced HEK293 cells. The values shown are representative of multiple independent experiments. DN, D-N mutant; KR; K-R mutant; WT, wild type; ZM, ZM1.