Fig. 5. Immuno-gold EM localization of PtIP3RN. (A,B) Dense labeling (gold grains) occurs in the layer around the collecting canals (CC) and represents the smooth spongiome (SS) (A), whereas the decorated spongiome (DS) shows only few gold grains (B). (C) Labeling also occurs directly adjacent to the lumen of the collecting canal. Bars, 0.1 µm.