Fig. 9. Effect of Ins(1,4,5)P₃-uncaging on local Ca²⁺ signaling at different distances from the ORS. Recordings of Ca²⁺ signals before (-0.36 seconds) and directly after the release of caged Ins(1,4,5)P₃ by UV light (+0.36 seconds, +0.72 seconds) show an increase in fluorescence after the activation of Ins(1,4,5)P₃ in close proximity to the ORS (arrow) (bar, 10 µm). Graph shows Ca²⁺ traces analyzed from different areas in the cell, which were adjusted to the minimal fluorescence value before UV illumination (F_min). During baseline recordings, spontaneous Ca²⁺ signals are observed. Illumination with UV light for 1 second (gray bar), i.e. uncaging of Ins(1,4,5)P₃, results in an increase of the Ca²⁺ signal from the area close to the upper vacuole (red) which is not seen to such an extent in the anterior (green) or posterior (blue) part of the cell.