Fig. 6. BFA-induced accumulation of GBF1 coincides with loss of COPI from peripheral VTCs that lie in close proximity to Sec31p-positive structures. (A) NRK cells treated with BFA (5 µg ml^-1) for various times (0; 10 seconds; 30 seconds and 1 minute) were fixed and processed for standard IF using anti-GBF1 rabbit antibody (9D2) and anti-ß-COP mouse antibodies (M3A5). White arrowheads mark puncta containing both GBF1 and ß-COP. (B) NRK cells treated with BFA (5 µg ml^-1) for 30 seconds were fixed and processed for standard IF using Alexa488-conjugated anti-GBF1 rabbit antibody (H-154) and rabbit anti-sec31p antibodies. Merged image shows a threefold magnification of boxed area in red and green channels. Bars, 5 µm.