Fig. 7. Nocodazole (NOZ) treatment stabilizes GBF1-positive peripheral VTCs. NRK cells expressing GFP-GBF1 were treated on ice for 15 minutes with either 20 µg ml^-1 NOZ or 0.5% DMSO. Following a 2 minute warm-up at 37°C, live cells were examined at 37°C on a Zeiss Axiovert 200M spinning disk microscope. Z-stacks of 6 slices each 1 µm thick were acquired continuously after addition of BFA (5 µg ml^-1). See supplementary material Movie 2. Still images from projected stacks at indicated times are shown. Bar, 20 µm.