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Figure 1


Fig. 1. Kinetics of RII{alpha} binding to and dissociation from the Golgi. Cells stably expressing RII{alpha}-GFP were photobleached with a high-intensity laser light and then the recovery of fluorescence was monitored by taking images at the indicated time points (in minutes). (A) The Golgi complex (outlined region) was subjected to a single bleach and recovery was analyzed by FRAP. Note that the centrosome was excluded from the bleached area. (B) The cytoplasm (area between the two encircled structures) was bleached repeatedly (every 4 minutes) to analyze RII{alpha}-GFP loss from the Golgi by FLIP. (C) Time-course of the evolution of Golgi-associated fluorescence during both FRAP and FLIP. Values are the mean ± s.e.m. of three different determinations.