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Figure 10


Fig. 10. PKA recruitment during Golgi reconstruction. Cells stably expressing GalTfase-CFP were incubated with 2 µg/ml BFA for 1 hour at 37°C and then allowed to recover for the indicated time periods. They were fixed and processed for immunofluorescence with anti-RII{alpha} antibody. Newly formed Golgi fragments containing (arrowheads) or not (arrows) associated RII{alpha} are indicated. Bar, 10 µm.