Fig. 5. Assessment of Golgi polarization after disassembly. Non-transfected HeLa cells were incubated (+) or not (-) for 80 hours with siRNA designed to inhibit the synthesis of RII. They were fixed and processed for indirect immunofluorescence with antibodies against the indicated endogenous Golgi proteins: giantin to label the cis-Golgi compartment and the TGN marker TGN46. Areas of colocalization (<10% total pixels) are white in the colocalization image. Bar, 10 µm.