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Figure 6


Fig. 6. Golgi disruption caused by PKA displacement. (A) Cells stably expressing galactosyltransferase-CFP (GalTfase) were transfected with cDNA encoding either a wild-type Ht31-GFP construct or the mutated form Ht31P-GFP. 72 hours post transfection, they were fixed and processed for immunofluorescence with anti-RII{alpha} antibody. Bar, 10 µm. (B) Cells expressing the indicated Ht31-GFP construct were fixed and processed for immunofluorescence with anti-giantin antibody. Golgi-associated fluorescence and the average number (± s.e.m.) of Golgi fragments per cell were determined (n=12).