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Figure 9


Fig. 9. Role of PKA in the organization of ER exit sites. (A) Presence of PKA at the ER exit sites. Cells overexpressing CFP-tagged forms of the indicated mutated versions of Sar1p were fixed and processed for immunofluorescence with antibodies to C{alpha}. (B) PKA displacement does not affect the distribution of ER exit sites. Cells incubated for 80 hours with siRNA to target either the human or the rodent form of RII{alpha} were fixed and processed for immunofluorescence with antibodies to Sec13p. Staining of the centrosome is indicated with arrowheads. Bars, 10 µm.