Fig. 3. Regulation of the clathrin-mediated EAAC1 internalization by syntaxin 1A. (A) Representative immunoblot and quantitative analysis showing that hypertonic medium containing 0.45 M sucrose increases EAAC1 surface expression and glutamate transport activity. ^**P<0.01 compared with the control (n=3). (B) The C6 glioma cells are transfected with ß-gal, wild-type dynamin (Dyn), or K44A dynamin (K44A) constructs. Representative immunoblot showing that the EAAC1 surface expression is decreased by transfection of dynamin but not by K44A dynamin. (C) Representative immunoblot of surface biotinylated-EAAC1 shows that Syn1A siRNA significantly rescues the dynamin-induced decrease in EAAC1 surface expression.