Fig. 7. KA stimulates EAAC1 internalization in primary cultured neurons. (A) Representative immunoblot and quantitative analysis of internalized EAAC1 upon KA stimulation in the primary cultured hippocampal neurons for various time showing that there is a constitutive internalization of EAAC1 that is significantly promoted by KA stimulation. ^*P<0.05, ^**P<0.01 compared with the cells without KA stimulation at identical time points (n=3). (B) Representative immunoblot and quantitative analysis show that stimulation with 10 µM KA decreases EAAC1 surface expression in the primary cultured hippocampal neurons. ^*P<0.05, ^**P<0.01 compared with the cells without KA stimulation (n=3).