Fig. 4. Adv infection saturates phosphorylation of downstream Akt effectors. (A) Cells were treated as described in Fig. 3, lysed at 12 hours and analyzed by western blot by probing with anti-pGSK-3/ß and anti-Akt-pSub. Experimental conditions are the same as those described in Fig. 3. 10% fetal bovine serum (FBS) stimulation for 15 minutes was used as the positive-control stimulus. 50 µg protein was loaded into each lane. (B,C) Adv-sensitized and IFN-sensitized (B) pGSK-3 and (C) Akt-pSub^42kDa bands were quantified by densitometry and normalized to the sensitized (Adv or IFN) 0-min band. Measurements are plotted as the mean of three biological replicates ± s.e.m. Changes are labeled as significant (^*) if P<0.05.