Fig. 5. Adv-infected are trapped near the plateau of a global Akt-survival dose-response curve. (A,B) Following Adv or IFN sensitization, cells were treated with TNF (100 ng/ml and 50 ng/ml, respectively), TNF + LY294002 (20 µM), TNF + insulin (100 nM), or TNF + LY294002 + insulin (B only). Conditions were chosen to generate a range of Akt activities. Measurements were taken at 12 hours for Akt activity and effector phosphorylation and at 24 hours for apoptosis as described in Fig. 3. (A) Cell survival versus fold activation of Akt activity. Akt activities were normalized to sensitized 0-min values from A. Akt measurements are the mean of six biological replicates. Survival measurements are 100% - the mean of six biological replicates. (B) Plot of survival (from A) versus effector phosphorylation levels quantified in Fig. 4. Solid red markers indicate IFN-sensitized conditions and open red markers indicate Adv-sensitized conditions (same as in A). (A,B) Sigmoid function and parameters were calculated as described in Materials and Methods. Transition steepness (T_s) and the upper and lower bounds of the 90% confidence interval (parentheses) are labeled on the graphs.