Fig. 2. Visualization of the three-dimensional structure of the actin cortex. Confocal images of fixed wild type (A,B) and myosin-II-null cells (C,D) in SB stained with Alexa Fluor 568-phalloidin to visualize F-actin. (B,D) Z-sections along the dotted lines in A and C, respectively. Arrowheads in A and B indicate actin arcs that compartmentalize the protrusion. Bars, 5 µm.