Fig. 8. Arl1Q72L localizes to the late Golgi in an arl3 mutant. (A) Mutant Arl1p localization in an arl3 mutant (upper panel). Different strains (as indicated) were stained with purified anti-Arl1p antibody and different ARL1 constructs were expressed in arl3-mutant yeast cells (lower panel); these ARL1 constructs were integrated into the leu2 locus in chromosomes. (B) Arl1Q72L colocalized with the late Golgi marker GFP-Sft2p in the arl3 mutant. Arl1Q72L and GFP-Sft2 were co-expressed in ARL3 and arl3 yeasts then stained with purified anti-Arl1p antibody and monoclonal anti-GFP antibody. Arrows indicate the colocalization of Arl1Q72L and GFP-Sft2.