Fig. 2. CDK7 and MAT1 subcellular distribution in early Drosophila embryogenesis. Cellular distribution of CDK7 (red signal) at different embryo developmental stages from syncytial blastoderm to gastrulation (a-d). DNA is shown in green. Note that the CDK7 signal is preferentially located in the cytoplasm at all stages. Distribution of the CAK complex protein MAT1 in early Drosophila development (e-h). The red signal is MAT1 after immunostaining with a polyclonal antibody (see Materials and Methods) and the green signal is DNA. (a,e) Early syncytial blastoderm embryos. (b,f) Syncytial blastoderm embryos at nuclear division 10. (c,g) Cellular blastoderm embryos. (d,h) Embryo during gastrulation.