Fig. 3. mAtg9 localizes to the trans-Golgi network. HEK293 cells were labelled with antibodies to mAtg9 and GM130 (A,B) or TGN46 (C,D). Cells in B and D were treated for 30 minutes with nocodazole (noc). Insets illustrate the colocalization after noc treatment. Bar, 10 µm. (E) Immunogold labelling of mAtg9 (10-nm gold, arrows) and GM130 (5-nm gold, arrowheads (left panel) in HEK293 cells. G, Golgi. Bars, 200 nm.