Fig. 2. Visualisation of MPM2-positive phosphoproteins in procyclic trypanosomes. Immunofluorescence images of T. brucei cytoskeletons co-labelled with the anti-phospho-epitope antibody MPM2 (green) and (A) ROD1, a specific marker for the extra-axonemal PFR structure; (B) BBA4, a specific marker for the proximal pole of both basal and probasal body structures; and (C) AB1, a specific marker for the central component of the flagella connector (red). Nuclear and kinetoplast DNA are labelled with DAPI (blue). The sections identified by the white boxes are enlarged in the top right hand corner of each panel to allow closer inspection of the co-labelling pattern observed. Bar, 10 µm.