Fig. 5. Detection of the FC on the old flagellum of cells not elongating a new flagellum and the flagellar sleeve is not an artefact of TbCHE2 RNAi. (A) Negatively stained whole-mount cytoskeleton showing a partial FC (asterisk in the inset) part way down the old flagellum (of) in a TbCHE2 RNAi-induced cell with two mature basal bodies (arrows). (B,C) The flagellar sleeve cannot be seen by scanning electron microscopy in a mutant that elongates a detached flagellum. The boxed region of B is shown at higher magnification in C. Bars 2 µm (A,B); 500 nm (C and inset in A).