Fig. 5. Loss of Apaf-1 protects the renal epithelium from tunicamycin. (A) Apaf-1^+/- (WT) and Apaf-1^-/- (KO) adult mice were injected intraperitoneally with 3 µg/gram tunicamycin. After 3 days, mice were sacrificed and kidneys were lysed or fixed for GRP78 expression or histological analyses, respectively. The induction of GRP78 in the kidneys was analyzed by western blotting. (B) Photomicrographs (original magnification, x50; upper panels and x200; lower panels) of kidney sections from Apaf-1^+/- or Apaf-1^-/- mice after tunicamycin injection. Thin sections were stained with hematoxylin and eosin. (C) TUNEL assay in kidneys from mice 48 hours after injection. Thin sections were stained for TUNEL assay or with DAPI. Experiments were repeated twice with similar results.