Fig. 3. SNX5-positive tubule extension and departure plays a role in the maturation of the macropinosome. HEK-GFP-SNX5 cell monolayers were serum starved overnight before being exposed to 100 ng/ml recombinant EGF 1 minute before the videomicroscopy was started. Time-lapse movies were recorded as in Fig. 1A. The interval between image capture was 10 seconds. Selected frames are presented with the time captured relative to the first frame recorded on the top right (minutes:seconds). Bar, 10 µm.