Fig. 3. Deletion of VPS1, DNM1 or FIS1 does not affect peroxisome positioning. Quantitative data on peroxisome positioning in budding cells were obtained by fluorescence microscopy of GFP-SKL-producing S. cerevisiae strains cultured on glucose (A,C,E,G,I) or in the presence of oleate (B,D,F,H,J). The number of fluorescent spots in each cell region (1-4) was counted from randomly taken fluorescence microscope images. Region 1 is the part of the mother cell opposite to the bud, region 2 is the central region in the mother cell, region 3 represents the region in the mother cell near the bud neck and region 4 is the developing bud (see A). For each sample, fluorescent spots were counted in 300 cells taken from two independent cultures (150 cells per culture). The frequency distributions of cell regions with different numbers of fluorescent spots are shown. Error bars represent the s.e.m.