Fig. 5. 3D model of a peroxisome in dnm1 vps1 cell. Characteristic 3D image of a peroxisome in a budding dnm1 vps1 cell, grown in the presence of oleate. The image is reconstituted based on a Z-stack of 22 CLSM images at 0.05 µm intervals. The figure demonstrates that in these cells typically one elongated organelle is present that protrudes into the bud (see also supplementary material Movie 4). Characteristically, the part of the organelle located in the mother cell is enlarged.