Fig. 9. Erasin is an ER stress-inducible protein. (A) HeLa cells were treated with 7 µM A23187, 2 mM DTT, 5 µM thapsigargin and 2 µg/ml tunicamycin for 16 hours. Cell lysates were collected and immunoblotted using polyclonal anti-erasin antibody 141, anti-BiP and anti-actin antibodies. (B) Levels of erasin protein (mean ± s.d.) seen after the different treatments relative to that of the untreated control were quantified from three independent experiments.