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Figure 10


Fig. 10. PI3K activation induces a hyperthickened epithelium in 3D organotypic cultures. (A) hematoxylin and eosin and BrdU stainings of untreated (nt), solvent-treated or 4-OHT-treated organotypic cultures of the Myr-p110{alpha}*-mER expressing cell clone 1 are shown. Treatment was initiated in 10-day-old cultures and continued for 15 days. (B) The bar graph represents the number of BrdU-positive cells per mm basement membrane. Error bars indicate s.e.m. *P<0.05; ***P<0.001; ns, not significant. (C) Solvent-treated or 4-OHT-treated organotypic cultures were analyzed by immunofluorescence for the presence of involucrin (a,b, green) and K10 (c,d, blue). Nuclei were counterstained with propidium iodide (red). The basement membrane is indicated as a dotted line. Bars, 50 µm.