Fig. 8. Expression of full-length DG and DG mutants in a pure DG^-/- MEpG cell line restored polarity and surface laminin in an 3D matrix of collagen-I-laminin-111. (A) Confocal immunofluorescent images taken at the center of colonies grown in collagen I (upper panel) or collagen-I-laminin-111 (middle panel). Samples were co-stained for ZO-1, 6 integrin and nuclei as described in Fig. 2A. Bottom panel shows laminin staining of a second group of colonies grown in collagen-I-laminin-111, visualized with Rhodamine-labeled secondary antibody (red). Cells are described in Fig. 7A. Bars, 10 µm. (B) Quantification of polarity in colonies grown in collagen I (C) or collagen-I-laminin-111 (C/L) using ZO-1 as a polarity marker. Results are shown as the average ± s.e.m. of 3-5 independent experiments, each with triplicate or quadruplicate counts. (^) or (^*)=P<0.001 for all paired combinations except with each other.