Fig. 7. (A) 14-3-3 binding to truncated MARK3. Wild-type (wt) or indicated truncated forms of GST-MARK3 (CT, residues 327-729; CTKA, residues 327-630) were expressed in HEK 293 cells, purified on glutathione-Sepharose and analysed by western blot with regards to total protein (GST) and 14-3-3 binding (14-3-3). (B) Subcellular localisation of truncated MARK3. Wild-type (wt) or indicated truncated forms of GFP-MARK3 were expressed in HEK 293 cells, which were fixed in 3% paraformaldehyde 24 hours post transfection. The GFP-fluorescence was analysed by confocal fluorescence microscopy. The cells shown are representative of images obtained in two separate experiments. Bars, 10 µm.